- Date: 02-12-10
- Location: Indiana-Gladstein FH - Bloomington, IN
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- ICON KEY:
| Place | Overall | Team | Squad | Athletes | Time | Score |
| 1. | 1 | Memphis | A | Richard Lowe, Alex Sweet, Stephan Ender, Abdi Guleed | 3:18.70 | - | |
| 2. | 2 | Indianapolis | A | Jerel Flournoy, Frederick Helm, Nick Brittain, Carl A Smith | 3:19.11 | - | |
| 3. | 3 | McKendree | A | Mike Mendez, Joshua Katam, Nick Thompson, Devin Williams | 3:20.74 | - | |
| 4. | 6 | Indiana | A | Logan Griffith, Joshua Moore, Joseph Holahan, Ryan Hindes | 3:24.47 | - | |
| 5. | 7 | Cincinnati | A | Ethan Freet, Brendon Lyshe, Allen Moore, Chase Van Cura | 3:24.74 | - | |
| 6. | 10 | Alabama-Huntsville | A | Aaron Lee, Richard Wilson, Cary Hale, Jaion Rayford | 3:27.85 | - |