- Date: 01-16-10
- Location: Iowa-Recreation Building - Iowa City, IA
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- ICON KEY:
Women's 400 Meters
- Finals: Heat #1
| Place | Overall | Name | Year | Team | Time | Score |
| 1. | 1 | Trusty, Rhonda-Kaye | SR-4 | Iowa | 57.06 | - | |
| 2. | 2 | Klier, Nikki | Unattached | 59.09 | - | ||
| 3. | 3 | Brockman, Miranda | SO-2 | Western Illinois | 1:01.32 | - | |
| 4. | 4 | Gladbach, Katie | FR-1 | Central Methodist | 1:05.38 | - | |
| 5. | 5 | Trokey, Jayna | SO-2 | Central Methodist | 1:06.38 | - | |
| 6. | 6 | Cummins, Jordyn | FR-1 | Central Methodist | 1:08.05 | - |