- Date: 02-19-10
- Location: Eastern Illinois-Lantz FH - Charleston, IL
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- ICON KEY:
| Place | Overall | Name | Year | Team | Time | Score |
| 1. | 1 | Dussett, Antwon | Unattached | 49.15 | - | ||
| 2. | 2 | Wiggan, Sean | SO-2 | Eastern Illinois | 49.48 | - | |
| 3. | 3 | Stull, Andrew | JR-3 | Indiana State | 50.71 | - | |
| 4. | 4 | Allen, Alexander | Florissant Valley CC | 50.73 | - | ||
| 5. | 10 | Murphy, Stephen | Rend Lake | 51.70 | - |