- Date: 04/02/10
- Location: Marquette - Milwaukee, WI
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- ICON KEY:
| Place | Team | Squad | Athletes | Time | Score |
| 1. | Wis.-Milwaukee | A | Florence Mafany, Kelsey Conrad, Kristi Schuette, Angie Kaufmann | 3:59.28 | 5 | |
| 2. | Marquette | A | Jenny Zahn, Alyssa Stevens, Kristin Pelzel, Christine Hansen | 4:03.37 | 3 | |
| 3. | Wis.-Milwaukee | B | Erika Hanson, Tanaiya Fisher, Beonca Mitchell, Shannon Brown | 4:12.46 | - |