- Date: 04/24/10
- Location: St. Mary's (Minn.) - Winona, MN
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- ICON KEY:
4 x 400 Relay
Heat 1
4 x 400 Relay
Heat 2
| Heat Pl. | Overall | Team | Squad | Athletes | Time | Score |
| 1. | 8 | Wis.-Stout | B | Jordan Evans, Ben Gavin, Matt Klopatek, David Smith | 3:38.04 | - | |
| 2. | 9 | St. Mary's (Minn.) | B | Benton Kodet | 3:43.22 | - | |
| 3. | 11 | Wis.-Stout | C | Mike Dannenbring, Wes Evers, Adam Sawinski, Sam Sujecki | 3:46.48 | - | |
| 4. | 12 | St. Mary's (Minn.) | A | Peter Borash | 3:47.54 | - | |
| 5. | 13 | UW - River Falls | C | 3:48.79 | - |
| Heat Pl. | Overall | Team | Squad | Athletes | Time | Score |
| 1. | 1 | Unattached | A | Alex Zeien, Indy Liljevall, Nick Zeien, Jared Brandenburg | 3:21.03 | - | |
| 2. | 2 | Wis.-Stout | A | Zach Anderson, Ethan Baumann, Sam Flood, Kendall Wade | 3:22.69 | - | |
| 3. | 3 | UW - River Falls | A | 3:23.58 | - | ||
| 4. | 4 | UW - Eau Claire | A | Corrie Floyd, Eric Pronschinske, Keegan Parrott, Tim Braunschweig | 3:25.01 | - | |
| 5. | 5 | Luther | A | Robert Matthiesen, Ryan Egan, Max Molzahn, JJ Cook | 3:30.88 | - | |
| 6. | 6 | Carleton | A | Ezra Velazquez, James Morrissey, Jordan Butler, Michael Austin | 3:33.91 | - | |
| 7. | 7 | UW - River Falls | B | 3:35.66 | - | ||
| 8. | 10 | Luther | B | Elliott Drake, Peter Kraus, Logan Langley, Kyle Buck | 3:44.45 | - |