- Date: 03/23 - 03/24/12
- Location: CSU-Pueblo - Pueblo, CO
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- ICON KEY:
| Place | Team | Squad | Athletes | Time | Score |
| 1. | Colorado St. | A | Shanetria Thomas, Meagan Robinson, Icenia Anderson, Bethany DeLong | 46.89 | - | |
| 2. | Northern Colorado | A | Natasha Bruins, Ashley Foster, Stephanie Ryken, Amoni Ashby | 48.09 | - | |
| 3. | CSU-Pueblo | A | Maya Richards, Vanessa Emerson, Marki Cook, Danita Wyatt | 49.28 | - | |
| 4. | Chadron State | A | Trae Patch, Stacee Craig, Meagan Nahrwold, Shelby Guy | 50.58 | - | |
| 5. | Colorado Mines | A | Michelle Rigsby, Erin Beach, Kayla Johnson, Kristen Rinedollar | 51.57 | - |