- Date: 03/31/12
- Location: U of Chicago - Chicago, IL
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- ICON KEY:
| Place | Team | Squad | Athletes | Time | Score |
| 1. | Loyola (Ill.) | A | Sophia Holy, Brigid Murray, Alyssa Pace-Patterson, Amy Persson | 51.12 | 10 | |
| 2. | U. of Chicago | A | Pam Yu, Madison Allen, Jaleesa Akuoko, Simone Aldredge | 51.17 | 8 | |
| 3. | Concordia (Ill.) | A | Lauren Zimmer, Danyale Worsham, Kyla Farquhar, Cassie Irwin | 52.41 | 6 | |
| 4. | Elmhurst | A | Jessica Cochiaro, Kim Stellmack, Zenya Yap, Alex Reid | 55.42 | 5 |