- Date: 5/4 - 05/05/12
- Location: Concordia (Ill.) - River Forest, IL
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- ICON KEY:
| Place | Team | Squad | Athletes | Time | Score |
| 1. | Concordia (Ill.) | A | Andrea Caldwell, Cassie Irwin, Leah Sellers, Danyale Worsham | 50.79 | 10 | |
| 2. | Wisconsin Lutheran | A | Amberly Weiglein, Zakkiyya Jones, Taylor Remstad, Tori Remstad | 51.29 | 8 | |
| 3. | Aurora | A | Loretta Johnson, Lauren Miller, Ainsley Richards, Courtney Roberts | 51.52 | 6 | |
| 4. | Edgewood | A | Katie Amelse, Cassandra Arnold, Kristina Santaga, Macalah Finstad | 52.04 | 5 | |
| 5. | Benedictine (Ill.) | A | Morgan Bartlett, Melissa Gregory, Nicole Millard, Natalie Kocks | 52.94 | 4 |