- Date: 5/4 - 05/05/12
- Location: Concordia (Ill.) - River Forest, IL
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- ICON KEY:
400 Meters
Heat 1
400 Meters
Heat 2
400 Meters
Heat 3
| Heat Pl. | Overall | Name | Year | Team | Time | Score |
| 1. | 8 | Sporie, Kat | JR-3 | Edgewood | 1:02.51 | 1 | |
| 2. | 15 | Vincenti, Anya | FR-1 | Aurora | 1:07.67 | - | |
| 3. | 18 | Holzer, Danielle | FR-1 | Lakeland | 1:08.61 | - | |
| 4. | 19 | Bowers, Jasmin | FR-1 | Concordia (Wis.) | 1:11.47 | - | |
| 5. | 20 | Bentsen, Rocky | JR-3 | Aurora | 1:12.04 | - |
| Heat Pl. | Overall | Name | Year | Team | Time | Score |
| 1. | 5 | Hennig, Jessica | SO-2 | Concordia (Wis.) | 1:01.58 | 4 | |
| 2. | 6 | Sapiro, Allison | FR-1 | Concordia (Ill.) | 1:01.61 | 3 | |
| 3. | 9 | Remstad, Tori | FR-1 | Wisconsin Lutheran | 1:03.17 | - | |
| 4. | 11 | Duffy, Barbara | SO-2 | Rockford | 1:03.96 | - | |
| 5. | 13 | Watson, Molly | FR-1 | Wisconsin Lutheran | 1:06.68 | - | |
| 6. | 14 | Mayer, Ashley | SO-2 | Aurora | 1:06.93 | - | |
| 7. | 16 | Espino, Ariana | FR-1 | Aurora | 1:07.84 | - | |
| 8. | 17 | Swain, Tracy | JR-3 | Aurora | 1:08.00 | - |
| Heat Pl. | Overall | Name | Year | Team | Time | Score |
| 1. | 1 | Santaga, Kristina | SO-2 | Edgewood | 59.77 | 10 | |
| 2. | 2 | Schwartz, Ellen | SO-2 | Wisconsin Lutheran | 1:00.70 | 8 | |
| 3. | 3 | Finstad, Macalah | FR-1 | Edgewood | 1:00.97 | 6 | |
| 4. | 4 | Frisque, Lauren | FR-1 | Concordia (Wis.) | 1:01.44 | 5 | |
| 5. | 7 | Remstad, Taylor | FR-1 | Wisconsin Lutheran | 1:02.43 | 2 | |
| 6. | 10 | Bartlett, Morgan | FR-1 | Benedictine (Ill.) | 1:03.80 | - | |
| 7. | 12 | Kocks, Natalie | FR-1 | Benedictine (Ill.) | 1:04.78 | - | |
| 8. | Johnson, Loretta | FR-1 | Aurora | NT | - |