4 x 400 Fresh-Soph
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| PL | TEAM | ATHLETES | TIME | TIME | TIME | TIME | TIME | TIME |
|---|---|---|---|---|---|---|---|---|
| 1 | Mainland | Marin Hawryluk, Taliyah Nixon, Autumn Demarest, Saraya Hinson | 4:54.00 | 5:08.70 | 4:59.88 | 4:18.72 | 4:42.24 | 4:51.06 |
| 2 | DeLand | Sydney Neira, Amy Nguyen, Sydney Bordeaux, Lilah Kersey | 5:04.19 | 4:00.31 | 3:57.27 | 4:55.07 | 4:36.82 | 5:43.74 |
| 3 | University (OC) | Lillie Pickens, Alea Smith, Shelsy Burgos Rosado, Sarahi Arroyo Canales | 5:33.84 | 5:27.17 | 5:20.49 | 6:00.55 | 6:23.92 | 5:40.52 |
| 4 | Lake Weir | 5:44.64 | 5:20.52 | 4:15.04 | 6:15.66 | 4:04.70 | 5:34.30 | |
| Father Lopez | Bianca Campos, Adelae Fulton, Marissa Stanberry, Frances Ginnochetti | DNS | DNS | DNS | DNS | DNS | DNS | |
| Olympia | Sandy Scott, Maddie Heald, Arlinda Roundtree, Korey Ardon | DNS | DNS | DNS | DNS | DNS | DNS |