4 x 400 Fresh-Soph
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| PL | TEAM | ATHLETES | TIME | TIME | TIME | TIME | TIME | TIME | TIME |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Mainland | Marin Hawryluk, Taliyah Nixon, Autumn Demarest, Saraya Hinson | 5:29.28 | 5:17.52 | 4:18.72 | 4:21.66 | 4:54.00 | 4:45.18 | 4:48.12 |
| 2 | DeLand | Sydney Neira, Amy Nguyen, Sydney Bordeaux, Lilah Kersey | 3:45.10 | 5:13.32 | 3:42.06 | 3:48.15 | 5:04.19 | 6:26.33 | 5:25.49 |
| 3 | University (OC) | Lillie Pickens, Alea Smith, Shelsy Burgos Rosado, Sarahi Arroyo Canales | 5:40.52 | 6:40.61 | 6:03.89 | 5:13.81 | 5:33.84 | 5:57.21 | 5:03.80 |
| 4 | Lake Weir | 5:58.43 | 4:39.16 | 4:15.04 | 5:48.09 | 5:44.64 | 5:13.63 | 5:06.73 | |
| Father Lopez | Bianca Campos, Adelae Fulton, Marissa Stanberry, Frances Ginnochetti | DNS | DNS | DNS | DNS | DNS | DNS | DNS | |
| Olympia | Sandy Scott, Maddie Heald, Arlinda Roundtree, Korey Ardon | DNS | DNS | DNS | DNS | DNS | DNS | DNS |